Two kinds of L-glutamine (Reanal-Hungary, Fluka-Switzerland) were applied in the spectrophotometric method of gamma dosimetry. Fluka L-glutamine was also applied as dosimeter of 10 MeV electron beam radiation. Two formulae were obtained for both glutamines for gamma radiation doses ranging from 0.76 kGy to 9.84 kGy. Another formula can be used in electron beam dosimetry for Fluka glutamine in doses ranging from 1.13 to 12.84 kGy. It has been shown that it is necessary to calibrate a new kind of L-glutamine dosimeters each time.