The results of electrophoretic separation of proteins (SDS-PAGE) revealed several protein bands from homogenate samples of irradiated and control adults of the confused flour beetle, Tribolium confusum DuVal. However, it was not possible to detect protein bands that show any shifts or separations between the irradiated and control beetles of the confused flour beetle. A remarkable reduction in the content of total proteins in irradiated adults was noted. Irradiation treatment altered the electrophoretic patterns and densities of proteins. The density of low molecular weight proteins (<16 kDa) increased, while that of high molecular weight proteins (>23 kDa) decreased. The alternations in total proteins of adult confused beetles are related to the dose of gamma radiation and the time elapsed after treatment. However, these clear changes in the electrophoretic pattern of protein fractions cannot be used to distinguish irradiated insects from non-irradiated ones as these alternations are not specific for irradiation.