NUKLEONIKA 2005, 50(Supplement 1):S63-S67
Pawe³ L. Urban1, Micha³ A. Baza³a1, Monika Asztemborska2, José L. Manjón3, Joanna Kowalska2, Gra¿yna Bystrzejewska-Piotrowska1, Dariusz Pianka1, Romuald Stêborowski1, Robert T. Kuthan4
1 Isotope Laboratory, Faculty of Biology,
Warsaw University, 1 Miecznikowa Str., PL-02-096 Warsaw, Poland
2 Laboratory of Applied Analytical Chemistry,
Faculty of Chemistry, Warsaw University, 1 Pasteura Str., 02-093 Warsaw, Poland
3 Departamento de Biología Vegetal,
Facultad de Biología, Universidad de Alcalá, 28871 Alcalá de Henares (Madrid),
Crtra. Madrid-Barcelona, Km. 33,600, Spain
4 Department of Medical Microbiology,
Medical University of Warsaw, 5 Cha³ubiñskiego Str., PL-02-004 Warsaw, Poland
A model species of saprophytic fungus, king oyster mushroom (Pleurotus eryngii), was cultivated
on barley substrate supplied with [Pt(NH3)4](NO3)2, under
well defined conditions. The samples of the collected fruiting bodies were digested and analyzed
for total platinum content by means of ICP-MS. The results proved that platinum is not accumulated
in the fruitbodies of Pleurotus eryngii for a wide range of Pt concentrations in the culture
substrate (100-1000 ppb Pt in 50 ml of water solution added to ca. 450 g of hydrated barley seeds per
container). Observable levels of Pt were only found in the fruitbodies obtained from the medium
contaminated with 10000 ppb (10 ppm) platinum solution. This demonstrates significant difference in
the effectiveness of platinum extraction in fungi and plants, which are capable to accumulate platinum
even when supplied at lower concentration (<500 ppb). It also shows different physiological pathways
of platinum and other elements which are easily accumulated in the fruitbodies of the same species.